Page 69 - New additives and ingredients in the formulation of aquafeeds

277.6
255.4
227.2
220.0
214.9
140.3
135.5
130.5
126.2
125.6
122.8
121.1
116.9
104.6
85.2
80.2
66.5
63.6
37.5
0 50 100 150 200 250 300
European pilchard
Atlan<c horse mackerel
Argen<ne shorAin squid
Longtail southern cod
Megrim
Haddock
Baird's slickhead
European hake
Redfish
Blue whi<ng
Pink cusk-­‐eel
Black scabbardfish
Patagonian grenadier
Blackbellied angler
Greenland halibut
Argen<ne hake
Roughsnout grenadier
Roughhead grenadier
Blue ling
19.2
143.9
19.6
52.4
89.9
412.3
138.9
53.9
55.2
369.7
340.9
321.7
149.8
41.8
11.9
18.3
138.6
321.7
107.2
0 100 200 300 400 500
Protease ac*vi*es in extracts
U/g viscera U/mg soluble protein
Common name FAO 3
α code Scientific name
Catches
(tonnes
/ year)
Viscera
(tonnes
/ year)
Middle East Atlantic
Megrim MEG Lepidorhombus whiffiagonis 249 20
Southwest Atlantic
Argentine hake HKP Merluccius hubbsi 13,421 1,074
Patagonian grenadier GRM Macruronus magellanicus 12,106 968
Longtail southern cod PAT Patagonotothen ramsayi 28,977 2,318
Pink cusk-eel CUS Genypterus blacodes 2,470 198
***Argentine shortfin squid SQA Illex argentinus 80,000 6,400
TOTAL 248,614 19,889
*In the case of blackbellied angler, data from the whole group of monkfish ( Lophiusspp.) captured by the
Spanish fleet in the Atlantic have been accounted.
**For these particular species no data have been found in the FAO statistical program.
***Data of argentine squid catches are estimates based on consultations to the fishing sector.
All these species were evaluated as potential sources of proteases. The
evaluation was oriented to assess the main types of proteases present in
the viscera, as well as the amount of enzyme activity present per g of fresh
tissue. The selection was focused to find sources of alkaline proteases,
considering their potential use as additive to enhance protein digestion in the
gut of juvenile fish, on which stomach digestion is still not well developed.
Preliminary evaluation of protease activity
Protease activities measured at pH 8.0 using haemoglobin as substrate in
crude visceral extracts are resumed in Figure 1.
Figure 1: Protease
activities in extracts
Use of purified extracts from fish viscera as an enzyme additive in feeds for juvenile marine fish 67