Page 78 - New additives and ingredients in the formulation of aquafeeds

Phases Equipment Materials & Reagents
Costs (€)
CEE PEE
4
Two-plates scale 79 79
Centrifuge 2,200 2,200
Centrifuge tubes 40 40
Filtration material
500 500
5
Glassware
Magnetic stirrer - 180
Ammonium sulphate - 26
6
Membrane dialysis - 103
Homogenization buffer* - 20.8
7
Freeze-dryer 1,500 1,500
Falcon tubes <1 <1
TOTAL 1.04 € 47.84 €
*Calculation of the homogenization buffer required for processing 1 kg of viscera.
Although Table 6 shows in detail the prices of all elements, when calculating
the totals only the reagents must be considered because the equipment
must be included into the amortization concept and materials would be
included into the operational costs. Also, it must be taken into account
that this cost would never be similar to that existing at industrial scale. The
total amounts represent the invested quantity, regarding reagents, by kg of
viscera or raw material used.
The estimated average yield of crude enzymatic extracts was 150 grams per
kg of viscera and the cost of the extraction process per gram was 0.007€. In
the case of purified enzyme extracts, the yields greatly varied with species
(Table 5), but considering an average value of 25 g/kg of viscera, the cost
would be increased up to 1.91 €/g. This great difference is the main reason
that oriented the use of CEE for the in vivo experiment.
76 Use of purified extracts from fish viscera as an enzyme additive in feeds for juvenile marine fish