Page 61 - New additives and ingredients in the formulation of aquafeeds

TRIAL 2: SOYBEAN MEAL REPLACED BY LUPIN AND CHICKPEA
HYDROLYSATES IN FATTEN DIETS FOR SEABREAM (Sparus aurata)
Summary:
Following with the aim of evaluating different innovative alternative
raw materials from the ones with animal origin to use in the aquaculture
nutrition in marine species, in this trial will be tested protein isolates from
different sources against commercial control diet.
Material and Methods:
Sea breamwith initial weight of 147 g to duplicate theirs weights were used.
These fishes were divided into 3 circular tanks of 500 L with 18 fishes per
tank. Approximately every 40 days from the beginning of the experiment
and until the end of it, each fish from each tank were weighed to calculate
the different growth rates and food conversion. At the beginning and
end of the test, different measures of length, weight, viscera, visceral fat
and whole fish sample and fillet for the calculation of the parameters for
biological and biochemical analysis were made. The starting trial conditions
were as follows:
Temperature (23-24ºC);
Feed Intake rate (1.5-1.6%);
Intakes/day: 2;
A total of 2 diets (Diet A - Diet B) based on a previously tested control
formula, with a level of protein/lipids 42/21 were developed and produced
(Photo 3). Both diets were designed with an inclusion of 15% protein
hydrolysate which is shown in Table 10.
Table 10. Experimental diets composition (% wet weight)
Diets Diet Control Diet A- Lupin
Hydrolysate
Diet B- Chickpea
Hydrolysate
Proteins 40.73 46.02 45.30
Fats 19.42 20.83 20.04
Ash 8.9 7.74 7.66
Moisture 5.69 6.45 6.52
Results and discussion:
From the third month, differences in the animal growth , with higher values​
for all diets compared to the control diet were observed; from this period,
only fishes fed by Diets A and B are significantly bigger than fish fed by
control diet (Table 11).
Plant protein isolates and hydrolysates as alternative to the animal protein in aquaculture diets 59